So you're using gel permeation chromatography (GPC) / size exclusion chromatography (SEC) but are experiencing problems with your system pressure. What do you do?

Separation Science, in collaboration with PSS, has developed an on-going series of tutorials looking at best practice and troubleshooting for GPC users. These tutorials are available free of charge requiring just a simple registration process.

The second tutorial in the series, titled 'Pressure Problems' provides strategies for understanding and rectifying pressure problems arising in your GPC/SEC system, to ensure that your analysis is optimized and that your system does not suffer damage as a result. To begin with you'll learn to identify the underlying problems.

Your standard GPC/SEC system should start off at approximately 95 bar pressure; however, circumstances can results in this pressure level becoming:

- significantly higher (e.g., 120 bar)
- significantly lower (e.g., 5 bar)
- increasingly higher over a loner period of time

This tutorial will look at the reasons for the above changes and provide strategies for correcting all three. It will describe the importance of identifying problems fast and offer best practice advice in terms of calibration, maintenance and cleaning.

To view this tutorial click on the link below:
http://learning.sepscience.com/form/gpc-sec-good-practice-troubleshooting-tutorials