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- Posts: 8
- Joined: Tue Jul 11, 2017 9:37 am
I am running a size exclusion chromatography in my HPLC. I am using chlorofrom as eluent at 1mL/min. The oven temperature is at 31C and the detector temperature is at 35C. The problem is that the baseline present at random times sudden drops (using RI detector). It is not a peak, but a drop in the baseline.
This is a normal chromatogram:
These are some wrong chromatograms:
I have an online degasser connected (membrane degasser), and I have also tried degassing the eluent with sonication or helium (still connected to the degaser), and have not seen any improve.
The pressure in the system does not seem to fluctuate (only from 963-967 psi), and also the temperature in the cell of the RI and the temperature in the oven are constant.
Any ideas?
Thanks in advance,
Anna