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- Posts: 656
- Joined: Tue Jul 05, 2005 7:45 am
- Location: Denmark
I have a problem with SEC of a protein product that also contains polysorbate. The polysorbate give a peak (micells?) exactly where the protein aggregates elute...
I was thinking about using a post-column derivatisation step and fluorescence detection? To test the idea I am thinking I could to use a t-union and then a small volume active mixer (Knauer) before the detector.
But what reagent should I use? Something that reacts fast and give good fluorescence response I guess. Or is the idea completely insane?
This would be run on a Waters Alliance with 474 detector.