Method validation for GPC method

Discussions about gel permeation chromatography / gel filtration chromatography / size exclusion chromatography

2 posts Page 1 of 1
Hi All

I hope you can help me. I have a GPC method that I need to validate. The method allows for the quantitative determination of the average molecular weight and molecular weight distribution in my samples.

I was thinking of doing the following for my method validation:

    Robustness - variation in mobile phase pH, buffer concentration of mobile phase, variation in column temperature, variation in inj vol, variation in flow rate, variation in UV wavelength.

      Method Precision - Repeatability and Intermediate Precision

      Are there any other "typical" validation parameters that i should consider? Are they any other available guidelines that can help me to better understand the requirements for validating a GPC method?

      Thanks in advance

      Mike
      Accuracy!

      Use a known standard or just some substance you have a documented Mw and see if you can determine the same Mw using your method.

      Best Regards
      Learn Innovate and Share

      Dancho Dikov
      2 posts Page 1 of 1

      Who is online

      In total there are 2 users online :: 0 registered, 0 hidden and 2 guests (based on users active over the past 5 minutes)
      Most users ever online was 599 on Tue Sep 18, 2018 9:27 am

      Users browsing this forum: No registered users and 2 guests

      Latest Blog Posts from Separation Science

      Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

      Subscribe to our eNewsletter with daily, weekly or monthly updates: Food, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

      Liquid Chromatography

      Gas Chromatography

      Mass Spectrometry