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- Posts: 2
- Joined: Wed Jul 22, 2015 10:48 am
I recently configured a new Agilent system to run size exclusion on various antibodies varying in size from roughly 50 kDa to 200 kDa. All works well; however I have never had to determine K and Alpha values before. I can find plenty of literature but am still struggling to understand how I may identify the correct values to allow calibration of the system and estimation of molecular weights.
Will one value suit all my samples or will each require alterations?
Any help would be appreciated.