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- Posts: 2
- Joined: Wed Dec 31, 2014 12:44 am
I have 2 SEC columns. They seem to be working fine (protein standards look good). However whenever I inject a specific protein into the columns, the retention time of the protein exceeds the permeation limit. This result suggests some sort of secondary interaction between this specific protein and the column, but I'm not sure what's causing it. In contrast all my other proteins seem to be flowing through the column at the appropriate retention times, suggesting no secondary interactions. Currently I'm looking through the literature to see if I can find anything about SEC troubles with this protein, but I haven't found anything yet.
Buffer: Phosphate and NaCl at pH 7.0
Any thoughts? Thanks.