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- Posts: 5
- Joined: Mon Mar 25, 2013 7:19 pm
I have a basic peptide pI ~11, Mw ~1500 Da that I want to buffer exchange using SEC column. I currently have G15 (MWCO>1500Da) and G10 sephadex (MWCO >700 Da). I am wondering if anyone can suggest me a type of buffer that would successfully elute this peptide? I try to stay with aqueous based buffer since this is for clinical application. I have tried phosphate, tris buffer, pH ranges from 5.5 to 8.5, and NaCl concentration up to 0.5 M, but this peptide seems to stick in the column. I am running isocratic mode. Another thing, this is my first experience with basic peptide. Any suggestions will be appreciated. Thank you.