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- Posts: 1
- Joined: Wed Mar 06, 2013 12:25 pm
I am currently looking for a method to separate a mixture of DNA fragments, meanly of 2 and 4 kb lenght. Normally DNA lenght separation is done with gel electroforese, however I do know that small DNA fragments like primers can be purified with a HPLC. For us, gel elektroforese is not possible as we cannot PCR our sample, and therefore we want to explore the possibilities of using an HPLC. Additionally, HPLC will allow us to fractionate our sample, which makes further analysis possible.
Therefore I was wondering if anyone has any experience with this, or can point me into a direction. What type of column would be most usefull, size exclusion, ion-exchange, etc?
Do you know any method described. Etc
With kind regards,
Rutger