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- Posts: 46
- Joined: Fri May 18, 2007 11:48 pm
I was interested to pose a question. Generally when it comes to separating large proteins from one another or large polymers from one another, it is generally accepted that Size Exclusion is the only way to do this. And that Reversed Phase separations and Normal Phase separations will not work.
But now I wonder why this should be so. We are all familiar with the On/Off mechanism by which large molecules separate on a column. So if, for example we had to separate two polymers and one was 700,000 and the other was 900,000 (same polymer just different sizes), shouldn't this be possible. Maybe we run a fairly standard gradient and find that they both come out around 35% organic, and then we go back and run a very very shallow gradient around that area.
Shouldn't it be possible to separate them. It seems like it should be possible but I've never seen this done.
Anyone have any experience with this??