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- Posts: 70
- Joined: Sun Sep 16, 2007 5:02 pm
im running a size exclusion chromatography method for a protein aggregate analysis.
column: silica matrix TOSOH column
mobile phase: 50 mM ammonium bicarbonate ph 7.0
sample matrix: 10 mM sodium acetate
capture wavelength: 215 nm
what i'm seeing is.. when i inject 10 mM sodium acetate there are peak at about 10 mins and there is a large dip in the baseline after about 40-45 mins. i've confirmed these are not because of protein carry over or any artifact. these are because of the acetate buffer and also the intensity of such interference increases with increase in acetate buffer concentrations.
these peaks interfere with my analysis and are very inconsistent in shape but always there.
any ideas on what is happening and what can be done?
thanks!