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- Posts: 25
- Joined: Thu Mar 31, 2011 9:22 pm
I am so glad that I found this forum, as I am new to HPLC world.
I got confused by these nomenclature. Are SEC/GFC/GPC nomenclature interchangable? This question has been brought to my attention many times at work, so I did some research on this.
SEC: size exclusion chromatography;
GPC: gel permeation chromatography;
GFC: gel filtration chromatography.
Typically, when an aqueous solution is used as mobile phase, it is called gel filtration chromatography (GFC); while gel permeation chromatography (GPC) is used when an organic solvent is used as a mobile phase.
If the column is used for separating proteins/peptides, or water soluble polymers (PEG, dextran, pullulan, etc), it would be SEC/GFC column. If it is for synthetic polymers (e.g., polystyrene, glyceride, etc.), which only dissovle in oil-like organic solvent (e.g., THF), it would be our GPC column.
Correct me if I made wrong.