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- Posts: 7
- Joined: Mon Jul 27, 2020 3:16 pm
I am testing IgG antibodies, and notice that the formulation buffer peak is eluting at the same time as LMWS peaks, essentially masking them so i cant really quantify LMWS %.
Is there any way to solve this problem?
Mobile Phase/Diluent is PBS pH 7.2, column is 300A 4.6x 300 mm
With isocratic for 16 minutes at 0.3 mL/min(recommended column flow rate)
The formulation buffer that antibody is in contains EDTA, PBS, and ammonium acetate.
Thank you.