Chromatography Forum

One of the benefits of Mixed-Mode Chromatography is compatibility with MS-detection. In mixed-mode columns, ion-pairing reagent is attached to the surface. This approach drastically increase capacity and selectivity of the column. In our opinion selectivity beats efficiency every time. Mixed-mode core-shell columns combine unique selectivity of mixed-mode stationary phases and ultra-high efficiency of core-shell particles. See our website for more applications
https://helixchrom.com/hplc-applications/
If you are struggling with method development there is no easier way than ask us to develop a method for you
Here is a good example of separation of 9 nucleotides on Coresep SB column:
https://helixchrom.com/compounds/guanosine-diphosphate/