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- Posts: 56
- Joined: Fri Aug 19, 2011 4:49 am
- Location: Australia
I'm following a semi-quantitative TLC protocol used for in-field screening of anti-malarials (GHPF Minilab) which uses visual comparison of standard and sample spots under UV light.
The problem is my eyes can't "see" any differences in spot intensity but when I coerce others into looking at my TLC plates, they can. It's getting tiresome having to find someone every time I need the plates read.
I've googled for techniques on how the inept might train themselves to recognise intensity differences but with no avail. Plenty of info on optimizing the method and using scanners but nothing for the incompetent.
We are using a CAMAG system with a viewing cabinet and turn off the lab lights. Wavelength is 254 nm and the plates are commercial pre-coated silica gel 60 F254.
Does anyone have any tips? Thanks