stuck sample onto cellulose

Off-topic conversations and chit-chat.

2 posts Page 1 of 1
Hi,
I attached a polyene molecule to cellulose. I was wondering if anyone had an idea how to verify that it's there.

I tried UV light. Not sure which wavelength it was but it's the kind that microbiologists use on surfaces i think, maybe for crosslinking too.

I also tried I2, but it stained blank cellulose the same. I tried permanganate stain and those looked the same too.

Next try will be FTIR, but I tried it on cellulose before and got a poor spectrum.
MestizoJoe
Analytical Chemist and Adventurer
Venture Industries
Spider-Skull Island
Do you want to know if is chemically bonded or just absorbed? Out there on Spider Skull Island Jonas may have left an old Beckman for UV analysis.
A polyene with conjugated double bonds should have a good UV-vis absorbance. If you want to confirm chemical bonding there may not be much difference between bonded and absorbed.
2 posts Page 1 of 1

Who is online

In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm

Users browsing this forum: No registered users and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry