Chromatography Forum

I hold chromatography as a hobby besides my career in synthetic chemistry.

Currently I'm building a completely from-scratch GC.
Complete with DIY:
-Injector (septum under a 1/8" swagelok Tee nut works great!)
-packed columns
-solid support/phases
-4-element TCD (made from 1/8 tee's)

Current development work is on:
-micropacked columns
-DIY megabore PLOT columns
-Corona discharge ionization detector

As it stands now it gives baseline sep. of propane, isobutane and butane on 50 cm packed column (paraffin on alumina support). Quite happy with that

Only original parts are septa and carrier flow control.

Back in the late 1990s/early 2000s our Pharmacia FPLC controllers at university died (electronics). There was no replacement available from GE Healthcare, because they wanted to promote their Äkta systems. So we hired a student who connected two pumps to a NI interface card and wrote a controller program for gradient generation / flow rate control using two of these pumps. As I moved house last week, I found the backup CD with this program
Sadly, I lost contact with my lab colleagues from these days, but I guess the pump system is still running for protein purification.

bunnahabhain wrote:
Back in the late 1990s/early 2000s our Pharmacia FPLC controllers at university died (electronics). There was no replacement available from GE Healthcare, because they wanted to promote their Äkta systems. So we hired a student who connected two pumps to a NI interface card and wrote a controller program for gradient generation / flow rate control using two of these pumps. As I moved house last week, I found the backup CD with this program
Sadly, I lost contact with my lab colleagues from these days, but I guess the pump system is still running for protein purification.

Would you be so kind to send me the program? I'm creating a Python library to control these old LKB Bromma / Pharmacia pumps, like the 2250. I'd be interested to see the implementation.