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- Posts: 24
- Joined: Thu Jun 18, 2009 9:13 am
- Location: RSA
thought this would be an interesting topic - if only to help me feel better about the most stupid thing i've done (i feel like a total idiot).
here's the story;
coleague brings me 2 samples with an HPLC method - wanting LC/MS spectra (to see if the compound is truely pure!)
HPLC mobile phase ---- 50mM KH2PO4:ACN
so I run a HPLC-PDA just to make sure the system compares to the results she got! perfect!
then, I connect to the MS never thinking for one second "hey there's phosphates in this mobile phase"
looked like snowflakes on the cone!
so I hope there are others out there that feel my pain.... or I at least made some of you laugh at my stupidity.
have a good day