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- Posts: 1
- Joined: Wed Jul 17, 2019 6:12 pm
I am trying to complete peptide hydrolysis with a big amount of samples at the same time (up to 30 samples).
Here are my constraints :
1. I don't want to recover the amino acid composition, I just want to cleave most of the bonds. I don't seek high hydrolysis/recovery quality, but rather the high throughput.
2. I don't have any specialized material at my disposal.
I saw a lot of papers referring to hydrolysis vials to perform the hydrolysis under vacuum, but hydrolysis vials are very expensive and are not appropriate for a high throughput experiment.
I was considering simply performing 6M HCl hydrolysis at 110°C for 24h, in borosilicate autosampler vials with screwed caps (PTFE/silicone septa), using a block heater.
However, the caps all slightly unscrew during the hydrolysis, and my samples evaporate.
Has anyone successfully tried to do peptide hydrolysis in screw-capped vials before? If so, what am I missing? Are my caps unsuitable?
Thank you very much for your answers !