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Triple Quad and MassLynx beginner, MS, MS2-Scan

Posted: Thu Feb 22, 2018 2:09 pm
by NoraStern
Hello,

I just started working with Triple Quad (Quattro Premier) and the software MassLynx. I have to tune perfectly ESI and the Analyser to detect quercetin. Therefore I use just 16 uM quercetin mixed with methanol. Unfortunately I never see stable peaks. It always looks like a crown with lots of small peaks - do you know what I mean? My professor just said "keep on tuning" but this is so frustrating. Nothing works. I see this crown when I use MS and MS2. But as I use collision gas, both peaks are gone. Even though I thought the sense of MS2 was to see peaks with this gas. When I look for fragments with the daughter scan, I can see them. But they aren't stable and well defined. Lots of crowns here aswell.

I hope somebody understands me and can help.

Re: Triple Quad and MassLynx beginner, MS, MS2-Scan

Posted: Mon Jun 25, 2018 7:54 am
by NoraStern
Spam deleted by admimistrator



Thank you, Alex. Maybe another person can help me out with my problem?

Re: Triple Quad and MassLynx beginner, MS, MS2-Scan

Posted: Mon Jun 25, 2018 10:28 am
by lmh
You really need some help from someone who actually uses this instrument, to check whether what you are seeing is normal or not. If you can't get help from a "real" person, an instrument user, can you post screen-shots here? Someone might be able to help. I haven't used this model myself.

Re: Triple Quad and MassLynx beginner, MS, MS2-Scan

Posted: Mon Jun 25, 2018 10:41 am
by NoraStern
Yes, I know, but unfortunately I don't have one. Have you seen my answer with the chromatogram in the other post?