I just started working with Triple Quad (Quattro Premier) and the software MassLynx. I have to tune perfectly ESI and the Analyser to detect quercetin. Therefore I use just 16 uM quercetin mixed with methanol. Unfortunately I never see stable peaks. It always looks like a crown with lots of small peaks - do you know what I mean? My professor just said "keep on tuning" but this is so frustrating. Nothing works. I see this crown when I use MS and MS2. But as I use collision gas, both peaks are gone. Even though I thought the sense of MS2 was to see peaks with this gas. When I look for fragments with the daughter scan, I can see them. But they aren't stable and well defined. Lots of crowns here aswell.

I hope somebody understands me and can help.