Triple Quad and MassLynx beginner, MS, MS2-Scan

Basic questions from students; resources for projects and reports.

4 posts Page 1 of 1

I just started working with Triple Quad (Quattro Premier) and the software MassLynx. I have to tune perfectly ESI and the Analyser to detect quercetin. Therefore I use just 16 uM quercetin mixed with methanol. Unfortunately I never see stable peaks. It always looks like a crown with lots of small peaks - do you know what I mean? My professor just said "keep on tuning" but this is so frustrating. Nothing works. I see this crown when I use MS and MS2. But as I use collision gas, both peaks are gone. Even though I thought the sense of MS2 was to see peaks with this gas. When I look for fragments with the daughter scan, I can see them. But they aren't stable and well defined. Lots of crowns here aswell.

I hope somebody understands me and can help.
Spam deleted by admimistrator

Thank you, Alex. Maybe another person can help me out with my problem?
You really need some help from someone who actually uses this instrument, to check whether what you are seeing is normal or not. If you can't get help from a "real" person, an instrument user, can you post screen-shots here? Someone might be able to help. I haven't used this model myself.
Yes, I know, but unfortunately I don't have one. Have you seen my answer with the chromatogram in the other post?
4 posts Page 1 of 1

Who is online

In total there are 8 users online :: 0 registered, 0 hidden and 8 guests (based on users active over the past 5 minutes)
Most users ever online was 599 on Tue Sep 18, 2018 9:27 am

Users browsing this forum: No registered users and 8 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry