Development beginner Bachelor Thesis

Basic questions from students; resources for projects and reports.

3 posts Page 1 of 1
Hello everyone, I'm starting my Bachelor's thesis in October and I'm a bit nervous. I am developing my first chromatography method and I am a bit overwhelmed with the column selection. My research has shown that RP columns are rather unsuitable for the molecules or require a lot of preparatory work. For "5-Ala", an RP column (C18) remains a rather poor choice. I am working on the method for a company and it is desired to measure all molecules with one method. I may also look into the HILIC method to possibly increase the team knowledge in the field of "polar analytics". The LogD values are below 0 and therefore "actually" suitable for HILIC. A test with C18 and caffeine could help. But I don't want to order unnecessary columns to reduce costs.

I would have ordered the Avantor HILIC-A,B,N columns and started tests with them.
Tomorrow i am going to start the firsts tests with a pentafluorophenyl column.
However, if anyone has some tips, tricks and favourite columns in mind, I would be very grateful for suggestions.

With kind regards

The four molecules to be analysed: Image
I'm coming at this from a "get the job done" POV rather than an academic perspective, but my first move would be to call the company that you're buying your columns from and talk to a technical person. And if they don't have good customer service and technical advice, find a different vendor.
Search here for your analytes and see what columns they recommend. ... ?s=type:LC

It may make selection easier.
The past is there to guide us into the future, not to dwell in.
3 posts Page 1 of 1

Who is online

In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm

Users browsing this forum: No registered users and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry