Page 1 of 1
Abscisic acid quantitation
Posted: Mon Nov 27, 2017 4:56 pm
by osp001
Can't seem to find a GC method to detect and quantitate abscisic acid that doesn't use derivatization.
Am I doomed to HPLC unless I want to derivatize, or does anyone have any suggestions?
Re: Abscisic acid quantitation
Posted: Mon Nov 27, 2017 7:20 pm
by tom jupille
Anything you analyze by GC must be volatile and/or thermally stable. Abscisic acid by itself is neither, hence the need for derivatization for GC.
Re: Abscisic acid quantitation
Posted: Tue Nov 28, 2017 5:40 pm
by lmh
If you do have access to LC-MS, it behaves quite well on reverse phase columns and ionises nicely by electrospray, so it's not too terrible being forced into liquid-world! Good luck!