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- Posts: 4
- Joined: Wed Jul 12, 2017 2:46 am
I'm not quite sure on how to determine the real sample concentration of a component. here is how I did the experiment, I use soxhlet extractor to extract antioxidant from flax seed. Before HPLC sample analysis, I first prepare a calibration curve (concentration Vs area) for the component. And then I took 100ul of oil from soxhlet extraction and dissolved in 900ul of alcohol. Then based on my calibration curve I got concentration of 3mg/ml. So, my question is, is this result (3 mg/ml) same as the real sample concentration? or do I have to consider a dilution factor for this? how do I calculate concentration of the real sample? and also i appreciate if you can suggest any material on this regard.
Thanks in advance,
Pardize