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- Posts: 3
- Joined: Wed Dec 09, 2015 6:08 am
Here is another situation, I was using same kind of column. But didn't do any activation. I used 10% Methanol for 30 minutes and after that ran standard solution of drug using Acetonitrile and PBS (0.1 M, 7 pH) 30:70 mobile phase.
This how I prepared the PBS,
NaH2PO4 (5.52 gm) 200 ml------Took 195 ml
Na2HPO4 (16.63 gm) 310 ml------ Took 305 ml
Total 500 ml + 500 (Distilled water) = 1000 ml PBS
After few minutes the pressure was too much high.
Later, I checked my column with another machine just to check using 10% Methanol the pressure was also high.
I need your guidance that how to activate my new column and how can I recover the used one.