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- Posts: 11
- Joined: Tue Aug 18, 2015 8:24 am
My lab is going to buy a HPLC. No one in lab has experience with HPLC.
The application is for separation of saccharide in samples e.g. glucose, fructose, maltose, maltotriose, higher saccharide, sugar alcohol. Thus, the detector will be Refractive Index Detector (RID).
What I know is, gradient elution can't be used in pair with RID so isocratic pump is enough for this application.
If there is enough budget to buy quaternary, which one is better? buy quaternary but use mostly for isocratic elution or just buy the isocratic pump?
Please help to give comment from the hardware's side e.g. the maintenance (which one is more difficult to maintain) or any negative impact to the quaternary pump because of continuous use at isocratic mode.
Thank you very much.