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Assay of Benzoyl Peroxide and Clindamycin Phosphate
Basic questions from students; resources for projects and reports.
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Hello, I am currently working on a acne gel which contains Benzoyl Peroxide and Clindamycin Phosphate as active ingredients. I am looking for one procedure to identify the compositions of both of these compounds together. Thank you in advance.
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Oooh, good luck with that one. Separating them using HPLC will likely not be a problem. It's the detecting that will be your issue. The peroxide has a benzene ring so you'll have a prayer with HPLC and a uv-light detector. That clindamycin doesn't have a good chromophore so a light detector might not work very well. You might have LCMS as your only option to get both in one shot.
Do you have the option to consider another technique? I just saw an article in one of my google alerts for getting benzoyl peroxide by NIR. Perhaps a technique like that would be a good approach for your problem?
Do you have the option to consider another technique? I just saw an article in one of my google alerts for getting benzoyl peroxide by NIR. Perhaps a technique like that would be a good approach for your problem?
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Hello rb6banjo and thank you for your reply, the thing is we do not have fancy equipment. We only have a standard spectrophotometer and HPLC. Ive looked all over the internet for methods with titration and I could not find any of that sort. If you have a method for only Benzoyl Peroxide by itself, do not hesitate to include it here.
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Have you checked "Google Scholar" for leads on how you might do this? It's a great - free-of-charge - way to find a lot of scientific information. At least it will point you to abstracts of papers that can get you on the right track.
I found a review in Analytical Chemistry:
Anal. Chem. 2007, 79, 4275-4294
that has the title "Pharmaceuticals and Related Drugs". In that paper, this paper is referenced as a chemiluminescence method for clindamycin.
(G44) Shao, X.; Xie, X.; Liu, Y.; Song, Z. J. Pharm. Biomed. Anal., 2006, 41 (2), 667-670.
I'm fairly confident you can find good mobile phase conditions to determine the benzoyl peroxide. The benzene ring is a fairly good chromophore in the uv.
I found a review in Analytical Chemistry:
Anal. Chem. 2007, 79, 4275-4294
that has the title "Pharmaceuticals and Related Drugs". In that paper, this paper is referenced as a chemiluminescence method for clindamycin.
(G44) Shao, X.; Xie, X.; Liu, Y.; Song, Z. J. Pharm. Biomed. Anal., 2006, 41 (2), 667-670.
I'm fairly confident you can find good mobile phase conditions to determine the benzoyl peroxide. The benzene ring is a fairly good chromophore in the uv.
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