RI vs ELS detectors for detection of polysaccharides

Basic questions from students; resources for projects and reports.

3 posts Page 1 of 1
I need to characterize dextrans and their non-UV active derivatives, and most of the literature I see uses refractive index detector. Is it possible to use evaporative light scattering? I always thought that it is more sensitive, stable and robust. What would you say?
Is it possible to use evaporative light scattering?
Yes: https://duckduckgo.com/?q=dextran+elsd&t=ffsb

ELSD pros:
- probably more sensitive
- less of an issue with temperature control
- compatible with gradients
ELSD cons:
- more complex (need for N2 source)
- harder to set up (more parameters to fuss with)
- mobile phase buffers must be volatile
- limited linear range

RI pros and cons pretty much the inverse.
-- Tom Jupille
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
Thanks. This is very helpful.
3 posts Page 1 of 1

Who is online

In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm

Users browsing this forum: No registered users and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry