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RI vs ELS detectors for detection of polysaccharides

http://www.chromforum.org/viewtopic.php?f=5&t=27033

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RI vs ELS detectors for detection of polysaccharides

Posted: Wed Jul 01, 2015 5:19 am
by HPLC_chainik
I need to characterize dextrans and their non-UV active derivatives, and most of the literature I see uses refractive index detector. Is it possible to use evaporative light scattering? I always thought that it is more sensitive, stable and robust. What would you say?

Re: RI vs ELS detectors for detection of polysaccharides

Posted: Wed Jul 01, 2015 6:22 am
by tom jupille
Is it possible to use evaporative light scattering?
Yes: https://duckduckgo.com/?q=dextran+elsd&t=ffsb

ELSD pros:
- probably more sensitive
- less of an issue with temperature control
- compatible with gradients
ELSD cons:
- more complex (need for N2 source)
- harder to set up (more parameters to fuss with)
- mobile phase buffers must be volatile
- limited linear range

RI pros and cons pretty much the inverse.

Re: RI vs ELS detectors for detection of polysaccharides

Posted: Wed Jul 01, 2015 7:59 pm
by HPLC_chainik
Thanks. This is very helpful.
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