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- Posts: 2
- Joined: Thu Feb 26, 2015 4:01 pm
Is it possible given:
- specs of the chromatography instrument
- peptide information (e.g. sequence)
- retention time
to calculate(estimate) the baseline width?
What I found so far is:
N = 16* (tr/w)^2 eq . (1)
since I have the retention times I wondered how to determine the value N so I can determine the baseline width w.
For N I found two different formula's that can make an estimate:
N = L/2dp, with L the column length and dp the particle diameter
and
N = L/H, with L again the column width and H the height of the plate.
Is it correct to calculate N based on one of these formula's and then filling it in equation (1), or am I on a completely wrong path?