Inconsistent rentention time HELP. URGENT~!!!

[richiekichi]

If you inject the same standard repeatedly do you still see the retention time drift?

Your differences in RT seem pretty negligible to me. Especially over such a large concentration range. But then I don't work in a regulated environment or with nice clean samples.

[paulinephoon]

If you inject the same standard repeatedly do you still see the retention time drift?

Your differences in RT seem pretty negligible to me. Especially over such a large concentration range. But then I don't work in a regulated environment or with nice clean samples.

Thanks for your opinion, richiekichi.

I lower my concentration and its range, so the difference RT wonldn't be so large.

Problem solved

[JMB]

Apart from the issues already mentioned, decreasing retention time with increasing concentration may be due to column overload.