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- Posts: 6
- Joined: Wed Jan 14, 2015 11:02 pm
I started with 1g of sample it went into 5ml reagent water after 30 minutes of shaking 5ml of MeCN was added. Mixture was shaken and then centrifuged. 50ul of the super went into 950ul of MeCN.
GC result is in ng/ml my calculation is result * dilution 1 * dilution 2 / sx weight
I haven been using 5 ml as my multiplier. Although the total volume is 10ml, I am taking my sample from the top layer so I would think that using 5 as my multiplier would make the most sense. When I use 10 as the multiplier my results are double than the expected value which would also support using the 5ml dilution as the multiplier.
I just want to make sure that i'm not missing a step, or not properly accounting for the dilutions.