Question about SPME, solubility, and volatility.

Basic questions from students; resources for projects and reports.

3 posts Page 1 of 1
I'm working with SPME to determine how well a certain steroid (androstenone) volatilizes in the air. When I have it in isopropyl alcohol I get a peak in GC-MS but when I have the same concentration in any type of oil (I've tried canola and Crisco) I do not see any peak. If I had to guess it is because androstenone is very soluble in oil compared to isopropyl alcohol so it is less likely to become volatile. Thanks in advance.
Lawborne wrote:
I'm working with SPME to determine how well a certain steroid (androstenone) volatilizes in the air. When I have it in isopropyl alcohol I get a peak in GC-MS but when I have the same concentration in any type of oil (I've tried canola and Crisco) I do not see any peak. If I had to guess it is because androstenone is very soluble in oil compared to isopropyl alcohol so it is less likely to become volatile. Thanks in advance.


That about sums it up.

Androstenone is a much larger molecules than is usual for SPME sampling and I would be interested to hear how well it performs since I am also working on airborne chemical signals: http://www.bpctrust.org/bioboundary-project.asp

Welcome to the forum.

Peter
Peter Apps
The octanol - air partition factor is not favorable for large and not very polar compounds in oils. I see less than 10% of the response when comparing naphthalene from water and naphthalene from waste oil when I do headspace (vacuum distillation in my case).
I see that Henry's law constants along with Kow and Koa data is available on Chemspider. It could give you a good starting point.
3 posts Page 1 of 1

Who is online

In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm

Users browsing this forum: No registered users and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry