by
Hollow » Thu May 08, 2014 6:58 pm
you're welcome
Millenium/Empower may be not that easy to begin but once you're into it gives you so many possibilities.
If not yet done, also use the help contents (F1). Sometimes it's a bit tricky to find the right infos but there's a lot of information built in. Normaly it will show you the topic related to the active window.
Just play a bit and try things out.
For this purpose, it's good to create something like a "training" project, where you can also import data from other "real" projects (right-cl>Copy to) and then just play around without messing up the real analysis.
Some known "beginners" mistakes (done myself...)
- when processing PDA data, you can't select a specific <processing method>. needs to be a <method set> (as from the previous post, the wavlength is defined there. otherwise you'd be trying to integrate a cube...)
- take some time to create a nice report with all the infos you want to have on your print out. Copy this to the other projects
- for referencing to your raw data, use the <result ID> or <injection ID>, these are unique numbers and with that you'll find your injection or sample set in a blink of an eye
- some nice feature to navigate is the <right click, view as...>
- hit <update> first, if you can't see what you've expected to see
- select the correct processing method type, when working with PDA data (needs to be <PDA> not <LC>), otherwise you won't see the spectral information for your peaks. And this type can't be changed later, so the processing method would have to be re-written
- use the <processing method wizard> until you're familiar where these parameter are written to. then wizard becomes obsolete for simple integration tasks (my opinion)
- many ways leads to "rome". find your own.
- for me, I normaly just use the "browse project" entry and do all my tasks from there (run samples, review, print etc.) and sometimes "configure system" for administration. Never used one of the bottom three entries.
- my way of processing (short, PDA data, no proc method yet)
-- load sample set into review
-- open the correct method set (see status bar which one is loaded)
-- go to "3d channels"-tab, select the PDA channel
-- in the toolbar, enter the wavelength needed, extract it
-- (use the "next injection" button to navigate trough the sample set)
-- manualy integrate the narrowest peak, click button for "peak width"
-- zoom in a region of baseline (as broad as possible and representative), click button for threshold
-- maybe set a miniumum hight (eg. 10x width of baseline ->LOQ)
-- right click, add integration events like "inhibit integration"*
-- toolbar integrate
-- select the peaks I want to label, right click, add name*
-- if everthing looks fine on all/most of the samples, save processing method
-- save method set
-- close review window
-- select sample set, right click, <process>
-- select method set of choice, ok
-> now there should be a result set with your chromatograms
-- if needed, load result set into review, do some manual re-integration, click quantitate (to re-lable the manual integrated ones), save <result>, close
-- print result set (should only include the newest (manualy intgr results)
*) don't remeber if this was already available in millenium or just came with Empower. otherwise go to processing meth window and fill in there.