So I have a breadth of oligos sized <50 nts. I'm trying to get my hands on a copy of "Handbook of Analysis of Oligonucleotides and Related Products", but in the mean time I could use some help getting started. I think the buffer systems are usually T(X)AA 100 mM (7.0-7.5 ph) and ACN run on a gradient over time.

Questions include, for someone who's never extensively used HPLC how do I go about writing a method? How do I go about choosiness correct solvent systems vs gradient vs time? And any advice on how to go from being indept to adept at it very quickly.

Thanks for the time and help,