-
- Posts: 3
- Joined: Fri Jan 24, 2014 4:57 pm
we have Agilent 1200 Binary Pump. How to get rid of air in the solvent line?
HPLC
Basic questions from students; resources for projects and reports.
4 posts
Page 1 of 1
-
- tom jupille
- Site Admin
-
- Posts: 4978
- Joined: Wed Aug 11, 2004 4:55 pm
- Location: Alamo, CA / USA
Read the user manual. The procedure is there.
-- Tom Jupille
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
-
- Posts: 3475
- Joined: Mon Jan 07, 2013 8:54 pm
- Location: Western Kentucky
If you do the normal purge and still see bubbles in the solvent line, then the bottle filter frit could be clogged, which will cause bubbles to form from cavitation in the solvent line. If the bubbles are worse the faster you run the pump this is probably the problem.
The past is there to guide us into the future, not to dwell in.
-
- Posts: 16
- Joined: Thu Jan 09, 2014 4:58 pm
Follow this common procedure to remove any bubbles in the lines :
1) immerse the two lines in a reservoir containing 100 % methanol .
2) open the pump purge .
3) adjust flow rate 2ml/min .
4) wash the two lines at the same time ( 50% : 50% ) with 100 % methanol for about 30 min .
ph/Amr Tarek
Instrumental Unit Head at Otsuka Pharmaceutical Company
1) immerse the two lines in a reservoir containing 100 % methanol .
2) open the pump purge .
3) adjust flow rate 2ml/min .
4) wash the two lines at the same time ( 50% : 50% ) with 100 % methanol for about 30 min .
ph/Amr Tarek
Instrumental Unit Head at Otsuka Pharmaceutical Company
4 posts
Page 1 of 1
Who is online
In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm
Users browsing this forum: No registered users and 1 guest
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm
Users browsing this forum: No registered users and 1 guest
Latest Blog Posts from Separation Science
Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.
Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.
- Follow us on Twitter: @Sep_Science
- Follow us on Linkedin: Separation Science
