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- Posts: 27
- Joined: Fri May 10, 2013 12:17 am
I have been spending so much time thinking about this but I'm still confused. I made a calibration curve for caffeine by injecting 5 ul of solution into the HPLC. The conc.'s I used were from 0.4 to 5 mg/ml. Then for my sample, I diluted .1517 g of a supplement powder (mixture of a lot of things) in 100 ml solvent, giving a conc. of 1.517 mg/ml. The amount injected was 5E-3, so I turned every concentration to mg on column. I need to get the caffeine amount in this 0.1517 g of powder by mg/g basis. How do I do that? Thanks so much.