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Rf value
Basic questions from students; resources for projects and reports.
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- tom jupille
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In thin layer chromatography or paper chromatography, Rf ("ratio of fronts") is the migration distance of a spot divided by the migration distance of the solvent front (both measured from the origin of the spot). If you want to relate it to other forms of liquid chromatography, Rf = 1/(1+k')
-- Tom Jupille
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
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I'm calculating my Rf value as follows:
distance dye traveled divided by the distance the solvent traveled
distance dye traveled divided by the distance the solvent traveled
- tom jupille
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that's correct.
-- Tom Jupille
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
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What does your calculation mean? It really confuses me.
- tom jupille
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-- Tom Jupille
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
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ratio of fronts....... I like that.......
I learned that Rf meant....
Factor of retardation back in them olden days.
I learned that Rf meant....
Factor of retardation back in them olden days.
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chromatographer1 wrote:
ratio of fronts....... I like that.......
I learned that Rf meant....
Factor of retardation back in them olden days.
Retention factor.
Analyte retention time / distance in HPLC or TLC is sensitive to the geometry of the column, run time, etc, . The retention factor (Rf) is a value for a compound that describes it's chromatography independantly of run, column length, etc. This value is useful for making comparisions while Rt is not.
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What is Rt?
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Rt=Retention time
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what is retention used for?
What category would it be used in the most?
What catetgory would it be used mainly for?
What category would it be used in the most?
What catetgory would it be used mainly for?
- tom jupille
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No, we're not going to do your homework for you.
Go through the tutorial here to find the answers:
http://www.lcresources.com/resources/getstart/
Go through the tutorial here to find the answers:
http://www.lcresources.com/resources/getstart/
-- Tom Jupille
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
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