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I’ve read a few posts on here about the ‘right’ and ‘wrong’ way to make up buffers. They are interesting, and apparently it is quite a controversial topic
I have a more academic question, regarding the maths of what’s going on.
For ease, convenience, and reproducibility we favour mixing calculated volumes rather than titration. I’ve also been advised that it’s far more reproducible to mix the buffer components rather than the salts (due to getting wet).
Is there a resource that explains the maths to work out given composition.
E.g. 10mM ammonium acetate can easily be prepared from 10mM ‘ammonia’ and 10mM acetic acid. But if I wanted acid/basic buffered how do I work out how much extra to add to a given pH.
In biology there are a tonne of tables that show exactly how much of x and y to mix to get a given pH but the waters and Agilent buffer prep guides just give the quantities of salt for an x mM solution.