﻿ Concentration calculation after HPLC analysis - Chromatography Forum

## Concentration calculation after HPLC analysis

Basic questions from students; resources for projects and reports.

### Concentration calculation after HPLC analysis

Hi there,
I will like some help confirming my calculations for the study below:

I have taken a sample with 86% moisture content, weighed out triplicate samples for HPLC analysis which averaged out at 504 mg.
The sample was extracted in 10 mL of solvent and 100uL of this extract was mixed with 900 uL to give a 1 mL sample which was injected into the HPLC.
The concentration after HPLC analysis was 127 mg/L and I have done some calculations where I got 18.2% w/w as the concentration of my analyte of interest. Is this correct?

### Re: Concentration calculation after HPLC analysis

Z = mg of analyte in the 10 mL extract

I'm assuming 100% of your analyte is extracted in that single, 10 mL shot.

Conc of analyte in the extract is Z/10 mg/mL

Z mg/10 mL x 0.1 mL/1 mL x 1,000 mL/L = 127 mg/L

Z mg = 127 x 10/(0.1 x 1,000) = 12.7 mg in the 10 mL extract

% Analyte in Sample = 12.7/504 x 100 = 2.5%

If you're concerned about the amount of analyte in the non-water matter, there's only 14% . So the non-water matter is 504 x 0.14 = 70.56 mg

%Analyte in the Non-Water Matter = 12.7/70.56 x 100 = 18%

### Re: Concentration calculation after HPLC analysis

Thanks a lot for your help

My pleasure.

### Who is online

In total there are 22 users online :: 0 registered, 0 hidden and 22 guests (based on users active over the past 5 minutes)
Most users ever online was 599 on Tue Sep 18, 2018 9:27 am

Users browsing this forum: No registered users and 22 guests

### Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.