Ion pair chromatography question

Basic questions from students; resources for projects and reports.

2 posts Page 1 of 1
Can someone explain how do ion-pair chromatography separate mixtures od acidic/basic compounds?
The (over)simplified explanation is that some of the ion-pair reagent (which is charged) sticks to the stationary phase. Analytes of opposite charge will then tend to be more strongly retained (opposite charges attract), while analytes of the same charge will tend to be more weakly retained (same charges repel).
-- Tom Jupille
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
2 posts Page 1 of 1

Who is online

In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm

Users browsing this forum: No registered users and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry