Hello people! I posted this question to this forum but just now noticed that it was in the wrong place. Anyway.

1. I'm one of many who are new to HPLC. I managed to do multilevel calibration without any issues and I'm happy with results. Now I'm playing around with real samples and this is where fun begins.

I'm familiar with this manual
https://www.agilent.com/cs/library/user ... anding.pdf but still can't put all the dots on i.

I want results of the compounds in ESTD%.

I take 200mg, total dilution is 2000 fold.
what is a multiplier?

2. Any recommendations for learning about integration? where is the line for peak integration to ensure data integrity?