LC-MS/MS for Chromatographers

Cost: US$ 249 per user for 12 months access
What is included: 41 video modules, 41 assessment tests, Certificate of Completion

Note: the first module is free access to illustrate the value of the course

What does it cover?
With the introduction of the mass spectrometer (MS) as a practical detector for a high-performance liquid chromatograph (LC or HPLC) in the early 1990s, LC-MS began to be used for routine applications. One form of LC-MS uses a tandem MS (LC-MS/MS), that has become the go-to instrument for quantitative analysis of drugs in biological matrices in the pharmaceutical laboratory. As they have become more widely used and understood, LC-MS and LC-MS/MS have found their way into environmental applications, synthetic chemistry, protein analysis, and many other areas. LC-MS/MS has moved from a technique available only to skilled experts in both HPLC and MS to a routine tool usable much as any other HPLC detector. You will find that this introductory course in LC-MS/MS will give you a good foundation of understanding the technique and how to use it in your daily work. We start out with an overview of different MS techniques, and focus in the majority of the course on the quadrupole mass spectrometer. You will learn how MS works, how ions are produced and fragmented. Sections focus on qualitative, as well as quantitative analysis. Each part of the process, calibration, tuning, sample preparation, HPLC method development, and MS method development are covered. Many tricks and techniques of practical use are presented.

Who should take this course?
This course is designed to provide basic knowledge and practical applications of LC-MS for liquid chromatographers. Most of the examples emphasize the most popular use of LC-MS/MS today: the analysis of drugs in metabolites in biological extracts. Although no previous experience with HPLC is necessary, the course will be especially valuable to those with some chromatography experience.

What will I get from this course?
You will learn how a mass spectrometer works, the advantages (and limitations) of adding mass spectrometric detection to your liquid chromatographic analyses, and how to use MS to approach separation and detection problems intractable by other methods. By understanding the central roles of the LC-MS interface and the sample ion, you will have a solid grounding on which to further build your LC-MS expertise.

The course was designed by John Dolan, Tom Jupille, and Lloyd Snyder. This class is taught by John Dolan, considered to be the one of the world’s experts in HPLC. He has written more than 300 user-oriented articles on HPLC troubleshooting over the last 30 years in addition to more than 100 peer-reviewed technical articles on HPLC and related techniques. His three books (co-authored with Lloyd Snyder), Troubleshooting HPLC Systems, Introduction to Modern Liquid Chromatography (3rd edn), and High-Performance Gradient Elution, are standard references on thousands of desks around the world. He also was intimately involved in the preparation of Snyder, Glajch, and Kirkland’s Practical HPLC Method Development (2nd edn) book. John is the author of Separation Science’s popular “HPLC Solutions” articles. From a method development standpoint, he has worked in a clinical chemistry department, managed an HPLC instrument manufacturer’s technical support laboratory, and written DryLab® software. He has taught HPLC training classes around the world to more than 10,000 students. His casual style and ability to get information across in a practical and understandable manner make him in high demand as a training instructor and conference speaker. The late Michael Alexander, Ph.D., was one of the early practitioner’s of LC-MS and LC-MS/MS, working with one multi-national and one startup pharmaceutical company as an LC-MS specialist. His combination of detailed understanding of all aspects of the MS, problem-solving capability, and easy communication of his vast knowledge are key factors in the continued success of this class.