Practical HPLC for Biopharmaceuticals

Although “small molecule” and “biopolymer” separations have traditionally been considered as separate activities, analysts in the biopharmaceutical industry regularly have to deal with both. Fortunately, the underlying principles of chromatography apply equally well in both situations when interpreted appropriately.

Cost: US$ 249 per user for 12 months access
What is included: 43 video modules, 43 assessment tests, Certificate of Completion

Note: the first module is free access to illustrate the value of the course

Who should take this course?
If you use LC on the job and would like a better understanding of how its various works techniques work and how and when to apply them to the analysis of biopharmaceuticals, “Practical HPLC for Biopharmaceuticals” is the course for you. It’s designed for pharmaceutical scientists who use HPLC as a regular part of their jobs and would like a better grounding in the technique, as well as enhancing knowledge and skills in alternative analytical solutions, and how and when to use them.

What does it cover?
“Practical HPLC for Biopharmaceuticals” explains chromatography in practical terms from the ground up. Here’s what you’ll learn in this online course:
    • The basics of isocratic and gradient HPLC
    • Reversed phase and ion exchange HPLC
    • HPLC equipment - from reservoirs/pumps to tubing/injectors to columns and detectors
    • Understanding and overcoming common column problems
    • Alternative modes: normal phase, HILIC and size exclusion
    • Quantitation
    • UHPLC
    • Performance qualification
    • Troubleshooting and diagnostics

What will I get from this course?
You will acquire a good understanding of the practicalities of HPLC and how it can be used in the analysis of biopharmaceuticals. What you learn will demystify the role of liquid chromatography in the analysis of biopharmaceuticals. You’ll find out the various roles that the different separation techniques play in the determination, characterization and quantitation of both small molecules and biopolymers. As well as conventional reversed phase HPLC you’ll will learn about ion-exchange, HILIC, size-exclusion chromatography and hydrophobic interaction chromatography - providing you with an analytical toolbox to apply to your own analytical challenges.

The course was designed by John Dolan, Tom Jupille, and Lloyd Snyder. This class is taught by Tom Jupille. Tom has been a practising chromatographer for more than 30 years, during which he has written more than 30 papers on chromatography and related subjects. He worked primarily in gas chromatography in the late ‘60s, switching to thin-layer chromatography in the early ‘70s and then to HPLC and ion chromatography in late ‘70s. His career has focused on instrument and column development and user support, providing a broad foundation of practical experience to call on as an instructor. He is probably best known as the moderator of the popular Chromatography Forum on-line chromatography discussion group.