Online chromatogram missing, HPLC-DAD
Posted: Thu Nov 08, 2018 1:40 pm
Hello,
I've done a few single runs on Agilent 1290 (binary pump). The instrument is not much in use. My sample was a plant extract (0.05 g/ml), centrifuged, filtrated (0.45 nm membrane filter), the injection volume 10 mcl. The mobile phase (1l): water/acetonitrile (6:4, v/v) (+0,8 ml H3PO4 and ca. 5 g SDS).
After a few runs, the online chromatogram started producing only vertical lines. There is no baseline during conditioning either. The front peak has a high intensity. The offline view, however, does show the chromatogram.
The software is: chemstation for lc 3d systems.
I'd very much appreciate it if I could get an answer about how to retrieve a normal online view of chromatograms and why I get to see only the vertical lines now?
I've done a few single runs on Agilent 1290 (binary pump). The instrument is not much in use. My sample was a plant extract (0.05 g/ml), centrifuged, filtrated (0.45 nm membrane filter), the injection volume 10 mcl. The mobile phase (1l): water/acetonitrile (6:4, v/v) (+0,8 ml H3PO4 and ca. 5 g SDS).
After a few runs, the online chromatogram started producing only vertical lines. There is no baseline during conditioning either. The front peak has a high intensity. The offline view, however, does show the chromatogram.
The software is: chemstation for lc 3d systems.
I'd very much appreciate it if I could get an answer about how to retrieve a normal online view of chromatograms and why I get to see only the vertical lines now?