ydna1977 wrote:
Hi ,
Not exactly what I was asking but OK
What happens if method states mean 2 standards and use these to quantify by calibration curve
It was exactly about what you asked
. Your calibration model seems wrong.
If you have only two standards at the same concentration, constructing a calibration line by linear regression will give you nonsense. I don't work with Empower, but I'd suppose if you select "linear" as calibration model it will use linear regression.
If you calibrate at one concentration only (no matter how many replicates) you're doing a one-point calibration. This is indeed a linear calibration where the second calibration point is the origin 0/0. Either select "one-point calibration" (or whatever it's called in Empower), this will of course also be a calibration with a calibration line. If you still want to use linear regression (which mathematically doesn't make much sense in this case, but of course it's possible to do)you need to force the origin through zero.