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I've read the Help section however it's a bit too technical for me at the moment so I was wondering if someone has time to explain the two parameters (and why/how use it at analysis)?
Thank you.
Discussions about chromatography data systems, LIMS, controllers, computer issues and related topics.
DavidHPLC wrote:
If I got it right, peakwidth will set the minimum width for peak recognition? As in, peaks wide less than X seconds will be ignored?
Not really. ApexTrack needs to smooth the signal before detecting peaks - otherwise it'd detect a lot of extra peaks (which are noise). Peak Width determines how much smoothing is applied:DavidHPLC wrote:
If I got it right, peakwidth will set the minimum width for peak recognition? As in, peaks wide less than X seconds will be ignored?
But when smoothing is excessive it may smooth out real peaks as well. For tall baseline-resolved peaks it won't matter much. But shoulders and round peaks are more subtle variations of the signal - they can easily be smoothed out to the point that they become undistinguishable. So your 2 poorly resolved peaks would look like 1 big peak to ApexTrack.Empower 3: Data Acquisition and Processing Theory Guide wrote:
The peak width sets the widths of the digital filters, which are used internally to obtain the smoothed, first and second derivative chromatograms. In ApexTrack, the role of the peak width parameter is solely to determine these filter widths.
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