-
- Posts: 301
- Joined: Tue Jul 12, 2016 4:47 pm
Our gradient method states that if a peak has a certain spectrum then its assigned as "X" compound, but this doesn't really account for how peaks can degrade or have other peaks co-elute over time and stressed conditions. I know co-elution can be proved by overlaying but with peaks that have mixed uv spectrum at different points of the peak, how can they be definitively termed? A purity test would obviously fail so I'm looking for opinions on how to make the call from "Its still Compound X but is breaking down" and "Because the overall spectrum is not like the method, it cant be called Compound X".