Connect Waters 474 to Chromeleon

Discussions about chromatography data systems, LIMS, controllers, computer issues and related topics.

20 posts Page 1 of 2
Hi,

I have connected a Waters 474 Fluorescence detector to my Chromeleon computer via the RS-232. It required a special 25-9 pin cable, and I have connection now.

I can control the detector from Chromeleon, but I cannot acquire any data. The command "acquisition on" is not in the programming menu :(

Any help is appreciated!!
Hi Mathias
With both Chromeleon (CM6 or CM7) you need a Analog / Digital converter. (UCI-50/100 or A2D). Did you install such a device?
Data acquisition trough the RS-232 is not supported.

Best regards,
Stefan Brand
Thermo Fisher Scientific
sbrand wrote:
Hi Mathias
With both Chromeleon (CM6 or CM7) you need a Analog / Digital converter. (UCI-50/100 or A2D). Did you install such a device?
Data acquisition trough the RS-232 is not supported.

Best regards,


Thank you for your reply!

I have not installed an A/D converter... I will look for one straight away!
We didn't have any in-house...

I assume that a Waters A/D converter will not work? (SAT/IN)
That's correct; the Waters Satin will NOT work with Chromeleon. The only Chromeleon compatible A/D Converter are UCI-50/100 (but obsolete from Thermo) and the A2D (actual modell).
Best regards,
Stefan Brand
Thermo Fisher Scientific
What driver do you use for the 474? I don't find it in the server configuration menu. I am asking because I have a 2475 and I'd like to know if I can control it using the same driver...

Thanks a lot
Jörg
bunnahabhain wrote:
What driver do you use for the 474? I don't find it in the server configuration menu. I am asking because I have a 2475 and I'd like to know if I can control it using the same driver...

Thanks a lot
Jörg


I am afraid that I do not know (or how to see that). It was our admin that hooked up the 474 to my computer.

We used the help menu to find out that we needed a special cable (Part no: 8914.0134) but there was not one word about a A/D-converter...
Funny: My Cm 6.8 lists only the following instruments:

Waters 515 Pump
Waters 600E Pump
Waters Acquity UPLC and UPLC H-Class System
Waters Alliance 2690(D)/2695(D) HPLC Modules
Waters Alliance 2790/2795 HPLC Modules
Waters 717plus Autosampler
Waters 996 and 2996 PDA Detectors
Waters 2487 UV Detector
Waters 2489 UV/Visible Detector and 2998 PDA Detector

No hit when searching for 474...
I don't know if it makes a difference, but we run on Chromeleon 7
Waters FLD 474 is supported in CM6 (at least in SR15) and in CM7 (7.2 MUb) and needs definitely in booth version a A/D converter.

At bunnahabhain: You may not have the latest SP for CM 6.8. You should upgrade to SR15; it's free of charge.
Stefan Brand
Thermo Fisher Scientific
I found another fluorescence detector in our "grave yard".

It is a Dionex RF2000. Do this detector also require an A/D converter?
RF2000 works using serial cable, no need for an A/D box.

@sbrand: I have SR15b but do not see the 474 driver. Nevermind, I don't have the 474 but the 2475...

Image
I installed the RF2000 via the RS232 and it doesn't work...

In the help-file it says that I need a firmware version 9 or higher. My firmware is version 3 :cry:
Your may right, there are some contradictory information. In the list of supported instruments for SR15b the FLD 474 is listed and marked as new. But i agree i there is no such driver available in the server configuration. For CM 7.2 the driver is available and can be selected. However, a A/D converter must be used in this case.
Stefan Brand
Thermo Fisher Scientific
Related to the RF2000:

Is it possible for me to update the firmware? Or do I need to get a service technician here?
20 posts Page 1 of 2

Who is online

In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm

Users browsing this forum: No registered users and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry