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- Posts: 1
- Joined: Thu Oct 09, 2014 2:55 am
I am new to the Shimadzu prominance system at my work place and no one has used the fraction collector before. I realise the Class VP software is well out of date but this is what I have to use as we cannot justify upgrading the software.
I have many years experience using Akta systems and I cannot believe how un-userfriendly the Class VP software is and how there is absolutely no manual for the FRC-10A fraction collector to use with the software.
What I am wanting to do is collect one peak from a semi-prep scale purification of a protein. Now I have tried to go about this a number of ways:
1 - In the Fraction collection tab in the method you can setup to collect either slope or level.
http://s983.photobucket.com/user/jaredraynes/media/Fractioncollection1.png.html?sort=3&o=1
Now what ever I seem to put into either of these values the Fraction collector will not start collecting. Even if I put 10 uV in. The weird thing also is that none of my chromatograms are in volts, only mAU so I really don't no what my volts are.
2 - In the time programme tab I select the collector module and select slope and put in a value, again 10 assuming the value means uV?? And again it will not collect any peak even very big ones.
http://s983.photobucket.com/user/jaredraynes/media/Fractioncollection2.png.html?sort=3&o=0
So how do I get it to collect only the peak I want and not everything or nothing????
I hope someone can help me because this is really pissing me off.
Regards,
Jared